Hemoglobin / Erythrocytes

Hemoglobin antibodies for flow cytometry

We offer antibodies to detect various types of hemoglobin. Each antibody can be used individually or combined in a panel with anti-HbS FITC, anti-panHb APC and anti-HbF Dy-410 for three lasers, eliminating the need for compensation.

 

Anti-hemoglobin S FITC
This is the first anti-hemoglobin S (HbS) antibody conjugate available for flow cytometry. For convenience, it’s conjugated with FITC, so it can be measured on all available flow cytometers.

The anti-HbS antibody recognizes the abnormal β-chain of HbS. Substitution of the amino acid valin for glutamic acid in the sixth position of the β-globin chain results in the formation of a βs subunit. Due to the mutated subunit βs, hemoglobin becomes unstable when no oxygen is bound, resulting in sickle shaped erythrocytes.

 

Figure 1. Flow cytometry analysis of whole blood stained with anti-hemoglobin S. Histograms: uninterrupted line represents HbShigh sample, interrupted line represents blanc sample.

 

Anti-pan hemoglobin APC
The murine pan hemoglobin (panHb) antibody recognizes the α-chain of human hemoglobin. This antibody can be combined with other antibodies to determine the percentage of erythrocytes containing pathological forms of hemoglobin compared to the total erythrocyte population.

Figure 2. Flow cytometry analysis of whole blood stained with anti-pan hemoglobin. Histograms: uninterrupted line represents stained sample, interrupted line represents blanc sample.

 

Anti-hemoglobin F Dy-410
The anti-hemoglobin F antibody recognizes the γ-chain of hemoglobin F (HbF). HbF is predominantly expressed by fetal erythrocytes and in some cases, HbF is also expressed by adult erythrocytes. These cells, so called F-cells, can represent up to 25 % of the total erythrocyte population.

Figure 3. Flow cytometry analysis of whole blood stained with anti-hemoglobin F. Histograms: uninterrupted line represents stained sample, interrupted line represents blanc sample.

 

Details on the available antibodies are listed here

Ready to measure hemoglobin with flow cytometry?

Place an order by sending an email to orders@iqproducts.nl or via the order form.

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IQ Products
Rozenburglaan 13a
9727 DL Groningen
The Netherlands

Tel +31 (0) 50 5757000
Fax +31 (0) 50 5757002
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